#Western blot ladder of protein sizes verification#

For most researchers, we generally recommend:Ī) ab116027 for proteins between 10 and 180 kDaī) ab116028 for larger proteins up to 245 kDaĬ) ab116029 for smaller proteins down to 3.5 kDaĭ) ab116029 or ab234592 for studying smaller and larger proteins at the same time. When choosing the right pre-stained protein ladder, the most important choice is based on the size of your protein of interest. Prestained ladders also can use highlight bands of a different color, to make it easier to identify different size bands. Protein ladders are most convenient to use when they are supplied ready to use, with no heating, dilution, or addition of reducing agent required before loading onto the gel. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel. Stronger blot persistence provides enhanced: Confidence after western blot processing. Increased brightness makes it easier to: Identify target proteins on the gel or blot. However, an unstained protein ladder can only be visualized following staining with Coomassie or a similar non-specific protein stain. Improved Dual Color Standards are brighter than the competition on both gels and blots. Using an unstained protein ladder is very useful when you need to accurately calculate the size of your protein. calculate the approximate size of your protein by overlaying an image of the membrane with the ladder with the image generated by antibody staining. verify transfer efficiency between gel and membrane (PVDF, nylon, or nitrocellulose) monitor protein separation during SDS-polyacrylamide gel electrophoresis J Cell Sci 127:124-36 (2014).Using a prestained protein ladder when running a western blot helps when you need to: Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains. Hypoxic 3D in vitro culture models reveal distinct resistance processes to TKIs in renal cancer cells. Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa. Lactate regulates autophagy through ROS-mediated activation of ERK1/2/m-TOR/p-70S6K pathway in skeletal muscle. Effect of sulfur-containing agrochemicals on growth, yield, and protein content of soybeans (Glycine max (L.) Merr). Publishing research using ab116027? Please let us know so that we can cite the reference in this datasheet.Īb116027 has been referenced in 5 publications. Review other protein ladders in the unstained and prestained protein ladder guide. Recommended loading: ~2 - 3 µl for western blots.Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.Ready-to-use: Supplied in a loading buffer for direct loading on gels.The ladder is supplied in gel loading buffer and is ready to use.ĭo not heat, dilute, add reducing agent before loading.

#Western blot ladder of protein sizes verification#

This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. Protein ladders are composed of a series of bands, which are highly purified polypeptides of different well-defined sizes. This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa).

Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa.